Increased Heat Resistance of Bacillus subtilis Spores in Oil.
نویسندگان
چکیده
منابع مشابه
Heat Resistance and Population Stability of Lyophilized Bacillus subtilis Spores.
Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the spores but did result in a slight decrease in population (</=0.3-logarithm red...
متن کاملResistance of Bacillus subtilis Spores to Inactivation
A statistical evaluation of viable count procedures utilized for obtaining treatment survival curve data for Bacillus subtilis NCTC 8236 spores is described. Within the various recovery conditions tested, incubation on nutrient agar containing 1% dextrose for 48 hr at 37 C was found to promote the highest count of viable spores surviving a variety of bactericidal treatments involving gamma irra...
متن کاملHeat shock proteins do not influence wet heat resistance of Bacillus subtilis spores.
Spores of Bacillus subtilis are significantly more resistant to wet heat than are their vegetative cell counterparts. Analysis of the effects of mutations in and the expression of fusions of a coding gene for a thermostable beta-galactosidase to a number of heat shock genes has shown that heat shock proteins play no significant role in the wet heat resistance of B. subtilis spores.
متن کاملHow moist heat kills spores of Bacillus subtilis.
Populations of Bacillus subtilis spores in which 90 to 99.9% of the spores had been killed by moist heat gave only two fractions on equilibrium density gradient centrifugation: a fraction comprised of less dense spores that had lost their dipicolinic acid (DPA), undergone significant protein denaturation, and were all dead and a fraction with the same higher density as that of unheated spores. ...
متن کاملEffects of minerals on resistance of Bacillus subtilis spores to heat and hydrostatic pressure.
Among Bacillus subtilis IFO13722 spores sporulated at 30, 37, and 44 degrees C, those sporulated at 30 degrees C had the highest resistance to treatments with high hydrostatic pressure (100 to 300 MPa, 55 degrees C, 30 min). Pressure resistance increased after demineralization of the spores and decreased after remineralization of the spores with Ca(2+) or Mg(2+), whereas the resistance did not ...
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ژورنال
عنوان ژورنال: Biocontrol Science
سال: 1998
ISSN: 1884-0205,1342-4815
DOI: 10.4265/bio.3.87